Journal: Scientific Reports

Article Title: Discovery of a kleptoplastic ‘dinotom’ dinoflagellate and the unique nuclear dynamics of converting kleptoplastids to permanent plastids

doi: 10.1038/s41598-019-46852-y

Figure Lengend Snippet: Diatom nuclear dynamics in Durinskia capensis observed with CLSM under autotrophic conditions. SYBR-Green or DAPI stained nucleus = Green, Mitotracker Green stained mitochondria = Yellow, Chlorophyll a autofluorescence = Red. ( A ) D. capensis ethanol-fixed on the sampling day (day 0). There are two round nuclei, a host dinoflagellate nucleus (Hn), which contains condensed chromosomes, and a diatom nucleus (En). ( B ) By day 7, the round diatom nucleus has disappeared from the D. capensis cell; instead string-shaped DNA signals can be observed alongside the plastids (arrow). ( C ) The Mitotracker Green-stained cell on day 7 indicates that the string-shaped DNA signals are from diatom mitochondria. ( D , E ) On day 35, no DNA signals from the diatom organelles can be observed in D. capensis with SYBR-Green ( D ) nor with Mitotracker Green ( E ). Sclae bar = 10 µm.

Article Snippet: For staining the nuclei of Durinskia spp., 500X working solution of SYBR Green I (Lonza, Basel, for living cells) or 125 μg/ml of DAPI (Carl Roth, Karlsruhe, for ethanol-fixed day 0 samples of D. capensis ) were used for a final concentration of 3% (v/v) or 6% (v/v), respectively.

Techniques: SYBR Green Assay, Staining, Sampling

Journal: Oncology Letters

Article Title: Potential synergistic effects of sorafenib and CP-31398 for treating anaplastic thyroid cancer with p53 mutations

doi: 10.3892/ol.2020.11377

Figure Lengend Snippet: Effects of CP-31398 on the number of SW579 cells in the cell cycle. (A) Cell cycle distribution. (B) Statistical analysis of cell cycle phases. SW579 cells were incubated with 0, 0.3 or 3 µM CP-31398 for 72 h. The CP-31398-treated cells were loaded into a Via-1-Casette and analyzed for DAPI dyes, using a fluorescence image cytometer (NucleoCounter NC-3000). Data were expressed as the means ± standard deviation. Student's t-test was used. **P<0.01.

Article Snippet: The stained cells were loaded into an NC-Slide A8 (ChemoMetec) and evaluated using a Fixed Cell Cycle-DAPI/DNA fragmentation assay protocol in the NucleoCounter NC-3000 image cytometer (ChemoMetec) ( 19 ).

Techniques: Incubation, Fluorescence, Cytometry, Standard Deviation